Tessellation analysis for STORM


In single molecule localization microscopy(SMLM), tessellation of the image using the centers of a specific molecule can reveal a wealth of information about the spatial arrangement of similar molecules. This helps in easily interpreting unique local attributes of an image and recurring patterns in the functionally active zones of the sample.

Tessellation based technique of image analysis harbors the potential to elucidate the link between  structure and function. Tessellations followed by K-Nearest neighbor search can not only reveal clusters of molecules that serve as a functional unit,  but also individual cluster properties like mean radius, number of localizations, cluster-cluster distances and much more. In our lab, the concept of tessellation is applied to interpret the organization of molecular organization and interactions of  biomolecular complexes sch as mTORC1.